研究業績 Publications

  1. Hashizume, O., Kawabe T., Funato, Y., and Miki, H.
    Intestinal Mg2+ accumulation induced by cnnm mutations decreases the body size by suppressing TORC2 signaling in Caenorhabditis elegans.
    Dev. Biol. 509, 59-69 (2024)
  2. Funato, Y., Hashizume, O., and Miki, H.
    Phosphatase-independent role of phosphatase of regenerating liver in cancer progression.
    Cancer Sci. 114, 25-33 (2023)
  3. Ryu, K., Yoshida, A., Funato, Y., Yamazaki, D., and Miki, H.
    PRL stimulates mitotic errors by suppressing kinetochore-localized activation of AMPK during mitosis.
    Cell Struct. Funct. 47, 75-87 (2022)
  4. Lohani, S., Funato, Y., Akieda, Y., Mizutani, K., Takai, Y., Ishitani, T., and Miki, H.
    A novel role for PRL in regulating epithelial cell density by inducing apoptosis at confluence.
    J. Cell Sci. 135, jcs258550 (2022)
  5. Funato, Y. and Miki, H.
    The emerging roles and therapeutic potential of cyclin M/CorC family of Mg2+ transporters.
    J. Pharmacol. Sci. 148, 14-18 (2022)
  6. Okumura, S., Konishi, Y., Narukawa, M., Sugiura, Y., Yoshimoto, S., Arai, Y., Sato, S., Yoshida, Y., Tsuji, S., Uemura, K., Wakita, M., Matsudaira, T., Matsumoto, T., Kawamoto, S., Takahashi, A., Itatani, Y., Miki, H., Takamatsu, M., Obama, K., Takeuchi, K., Suematsu, M., Ohtani, N., Fukunaga, Y., Ueno, M., Sakai, Y., Nagayama, S., and Hara, E.
    Gut bacteria identified in colorectal cancer patients promote tumourigenesis via butyrate secretion.
    Nat. Commun. 12, 5674 (2021)
  7. Funato, Y., Yamazaki, D., Okuzaki, D., Yamamoto, N., and Miki, H.
    Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure.
    Nat. Commun. 12, 3683 (2021)
  8. Huang, Y., Mu, K., Teng, X., Zhao, Y., Funato, Y., Miki, H., Zhu, W., Xu, Z., and Hattori, M.
    Identification and mechanistic analysis of an inhibitor of the CorC Mg2+ transporter.
    iScience 24, 102370 (2021)
  9. Yamazaki, D., Hashizume, O., Taniguchi, S., Funato, Y., and Miki, H.
    Role of adenomatous polyposis coli in proliferation and differentiation of colon epithelial cells in organoid culture.
    Sci. Rep. 11, 3980 (2021)
  10. Gehring, K. and Miki, H.
    Phosphatase, pseudo-phosphatase, or both? Understanding PRL oncogenicity.
    Br. J. Cancer 124, 1035–1036 (2021)
  11. Huang, Y., Jin, F., Funato, Y., Xu, Z., Zhu, W., Wang, J., Sun, M., Zhao, Y., Yu, Y., #Miki, H., and #Hattori, M. (#: co-correpondence)
    Structural basis for the Mg2+ recognition and regulation of the CorC Mg2+ transporter.
    Science Advances 7, eabe6140 (2021)
  12. Wu, Y., Funato, Y., Meschi, E., Jovanoski, K., Miki, H., and Waddell, S.
    Magnesium efflux from Drosophila Kenyon Cells is critical for normal and diet-enhanced long-term memory.
    eLife 9, e61339 (2020)
  13. Funato, Y., Yoshida, A., Hirata, Y., Hashizume, O., Yamazaki, D., and Miki, H.
    The oncogenic PRL protein causes acid addiction of cells by stimulating lysosomal exocytosis.
    Dev. Cell 55, 387-397 (2020)
  14. Kozlov, G., Funato, Y., Chen, Y.S., Zhang, Z., Illes, K., Miki, H., and Gehring, K.
    PRL3 pseudophosphatase activity is necessary and sufficient to promote metastatic growth.
    J. Biol. Chem. 295, 11682-11692 (2020)
  15. Ito, S., Nada, S., Yamazaki, D., Kimura, T., Kajiwara, K., Miki, H., and Okada, M.
    p18/Lamtor1-mTORC1 signaling controls development of mucin-producing goblet cells in the intestinal epithelium.
    Cell Struct. Funct. 45, 93-105 (2020)
  16. Matsushita, M., Nakamura, T., Moriizumi, H., Miki, H., and Takekawa, M.
    Stress-responsive MTK1 SAPKKK serves as a redox sensor that mediates delayed and sustained activation of SAPKs by oxidative stress.
    Science Advances 6, eaay9778 (2020)
  17. Hashizume, O., Funato, Y., Yamazaki, D., and Miki, H.
    Excessive Mg2+ Impairs Intestinal Homeostasis by Enhanced Production of Adenosine Triphosphate and Reactive Oxygen Species.
    Antioxid. Redox Signal. 33, 20-34 (2020)
  18. Dóka, É., Ida, T., Dagnell, M., Abiko, Y., Luong, N.C., Balog, N., Takata, T., Espinosa, B., Nishimura, A., Cheng, Q., Funato, Y., Miki, H., Fukuto, J.M., Prigge, J.R., Schmidt, E.E., Arnér, E.S.J., Kumagai, Y., Akaike, T., and Nagy, P.
    Control of protein function through oxidation and reduction of persulfidated states.
    Science Advances 6, eaax8358 (2020)
  19. Kojima, T., Funato, Y., and Miki, H.
    Phosphatase of regenerating liver sensitizes MET to functional activation by hepatocyte growth factor.
    Biochem. J. 476, 1419-1431 (2019)
  20. Yamazaki, D., Hasegawa, A., Funato, Y., Tran, H.N., Mori, M.X., Mori, Y., Sato, T., and Miki, H.
    Cnnm4 deficiency suppresses Ca2+ signaling and promotes cell proliferation in the colon epithelia.
    Oncogene 38, 3962-3969 (2019)
  21. Funato, Y. and Miki, H.
    Molecular function and biological importance of CNNM family Mg2+ transporters.
    J. Biochem. 165, 219-225 (2019)
  22. Chen, Y.S., Kozlov, G., Fakih, R., Funato, Y., Miki, H., and Gehring, K.
    The cyclic nucleotide-binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+ efflux activity.
    J. Biol. Chem. 293, 19998-20007 (2018)
  23. Yoshida, A., Funato, Y., and Miki, H.
    Phosphatase of regenerating liver maintains cellular magnesium homeostasis.
    Biochem. J. 475, 1129-1139 (2018)
  24. Funato, Y., Furutani, K., Kurachi, Y., and Miki, H.
    CNNM proteins are Na+/Mg2+ exchangers playing a central role in transepithelial Mg2+ (re)absorption.
    J. Physiol. 596, 743-746 (2018)
  25. Matsui, Y., Funato, Y., Imamura, H., Miki, H., Mizukami, S., and Kikuchi, K.
    Visualization of long-term Mg2+ dynamics in apoptotic cells using a novel targetable fluorescent probe.
    Chem. Sci. 8, 8255-8264 (2017)
  26. Funato, Y., Yamazaki, D., and Miki, H.
    Renal function of cyclin M2 Mg2+ transporter maintains blood pressure.
    J. Hypertens. 35, 585-592 (2017)
  27. Gulerez, I., Funato, Y., Wu, H., Yang, M., Kozlov, G., #Miki, H., and #Gehring, K. (#: co-correpondence)
    Phosphocysteine in the PRL-CNNM pathway mediates magnesium homeostasis.
    EMBO Rep. 17, 1890-1900 (2016)
  28. Ishii, T., Funato, Y., Hashizume, O., Yamazaki, D., Hirata, Y., Nishiwaki, K., Kono, N., Arai, H., and Miki, H.
    Mg2+ Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans.
    PLoS Genet. 12, e1006276 (2016)
  29. Yamazaki, D., Funato, Y., Miyata, H., Ikawa, M., and Miki, H.
    Complementary role of CNNM2 in sperm motility and Ca(2+) influx during capacitation.
    Biochem. Biophys. Res. Commun. 474, 441-446 (2016)
  30. Yamazaki, D., Miyata, H., Funato, Y., Fujihara, Y., Ikawa, M., and Miki, H.
    Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction.
    J. Cell Sci. 129, 1940-1949 (2016)
  31. Hirata, Y., Funato, Y., and Miki, H.
    Basolateral sorting of the Mg(2+) transporter CNNM4 requires interaction with AP-1A and AP-1B.
    Biochem. Biophys. Res. Commun. 455, 184-189 (2014)
  32. Funato. Y,, Yamazaki, D., Mizukami, S., Du, L., Kikuchi, K., and Miki, H.
    Membrane protein CNNM4-dependent Mg2+ efflux suppresses tumor progression.
    J. Clin. Invest. 124, 5398-5410 (2014)
  33. Yugi, K., Kubota, H., Toyoshima, Y., Noguchi, R., Kawata, K., Komori, Y., Uda, S., Kunida, K., Tomizawa, Y., Funato, Y., Miki, H., Matsumoto, M., Nakayama, K.I., Kashikura, K., Endo, K., Ikeda, K., Soga, T., and Kuroda, S.
    Reconstruction of insulin signal flow from phosphoproteome and metabolome data.
    Cell Rep. 8, 1171–1183 (2014)
  34. Hirata, Y., Funato, Y., Takano, Y., and Miki, H.
    Mg2+-dependent interactions of ATP with the cystathionine-β-synthase (CBS) domains of a magnesium transporter.
    J. Biol. Chem. 289, 14731-14739 (2014)
  35. Funato, Y. and Miki, H.
    Reversible oxidation of PRL family protein-tyrosine phosphatases.
    Methods 65, 184-189 (2014)
  36. Yamazaki, D., Funato, Y., Miura, J., Sato, S., Toyosawa, S., Furutani, K., Kurachi, Y., Omori, Y., Furukawa, T., Tsuda, T., Kuwabata, S., Mizukami, S., Kikuchi, K., and Miki, H.
    Basolateral Mg2+ Extrusion via CNNM4 Mediates Transcellular Mg2+ Transport across Epithelia: A Mouse Model.
    PLoS Genet. 9, e1003983 (2013)
  37. Funato, Y., Hayashi, T., Irino, Y., Takenawa, T., and Miki, H.
    Nucleoredoxin regulates glucose metabolism via phosphofructokinase 1.
    Biochem. Biophys. Res. Commun. 440, 737-742 (2013)
  38. Yamazaki, D., Itoh, T., Miki, H., and Takenawa, T.
    srGAP1 regulates lamellipodial dynamics and cell migratory behavior by modulating Rac1 activity.
    Mol. Biol. Cell 24, 3393–3405 (2013)
  39. Ishii, T., Funato, Y., and Miki, H.
    Thioredoxin-related protein 32 (TRP32) specifically reduces oxidized phosphatase of regenerating liver (PRL).
    J. Biol. Chem. 288, 7263–7270 (2013)
  40. Terabayashi, T., Sakaguchi, M., Shinmyozu, K., Ohshima, T., Johjima, A., Ogura, T., Miki, H., and Nishinakamura, R.
    Phosphorylation of Kif26b promotes its polyubiquitination and subsequent proteasomal degradation during kidney development.
    PLoS One 7, e39714 (2012)
  41. Miki, H., and Funato, Y.
    Regulation of intracellular signalling through cysteine oxidation by reactive oxygen species.
    J. Biochem. 151, 255–261 (2012)
  42. Morinaka, A., Funato, Y., Uesugi, K., and Miki, H.
    Oligomeric peroxiredoxin I is an essential intermediate for p53 to activate MST1 kinase and apoptosis.
    Oncogene 30, 4208-4218 (2011)
  43. Morinaka, A., Yamada, M., Itofusa, R., Funato, Y., Yoshimura, Y., Nakamura, F., Yoshimura, T., Kaibuchi, K., Goshima, Y., Hoshino, M., Kamiguchi, H., and Miki, H.
    Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 and growth cone collapse.
    Science Signal. 4, ra26 (2011)
  44. Yoshimura, Y., and Miki, H.
    Dynamic regulation of GEF-H1 localization at microtubules by Par1b/MARK2.
    Biochem. Biophys. Res. Commun. 408, 322-328 (2011)
  45. Müller, L., Funato, Y., Miki, H., and Zimmermann, R.
    An interaction between human Sec63 and nucleoredoxin may provide the missing link between the SEC63 gene and polycystic liver disease.
    FEBS Lett. 585, 596-600 (2011)
  46. Funato, Y., Terabayashi, T., Sakamoto, R., Okuzaki, D., Ichise, H., Nojima, H., Yoshida, N., and Miki, H.
    Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive dishevelled protein.
    Curr. Biol. 20, 1945-1952 (2010)
  47. Hayashi, T., Funato, Y., Tearabayashi, T., Morinaka, A., Sakamoto, R., Ichise, H., Fukuda, H., Yoshida, N., and Miki, H.
    Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of Flightless-I to Myeloid differentiation primary response gene (88).
    J. Biol. Chem. 285, 18586-18593 (2010)
  48. Uchiyama, Y., Sakaguchi, M., Terabayashi, T., Inenaga, T., Inoue, S., Kobayashi, C., Oshima, N., Kiyonari, H., Nakagata, N., Sato, Y., Sekiguchi, K., Miki, H., Araki, E., Fujimura, S., Tanaka, S., and Nishinakamura, R.
    Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme.
    Proc. Natl. Acad. Sci. U.S.A. 107, 9240-9245 (2010)
  49. Yoshimura, Y., Terabayashi, T., and Miki, H.
    Par1b/MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation.
    Mol. Cell. Biol. 30, 2206-2219 (2010)
  50. Funato, Y., and Miki, H.
    Redox regulation of Wnt signalling via nucleoredoxin.
    Free Radic. Res. 44, 379-388 (2010)
  51. Boles, M. K., Wilkinson, B. M., Wilming, L., Liu, B., Probst, F. J., Harrow, J., Grafham, D., Hentges, K. E., Woodward, L. P., Maxwell, A., Mitchell, K., Risley, M. D., Johnson, R., Hirschi, K., Lupski, J. R., Funato, Y., Miki, H., Marcin-Garcia, P., Mattews, L., Coffey, A. J., Parker, A., Hubbard, T. J., Rogers, J., Bradley, A., Adams, D. J., and Justice, M. J.
    Discovery of candidate disease genes in ENU-induced mouse mutants by large-scale sequencing identifies a splice-site mutation in nucleoredoxin.
    PLoS Genet. 5, e1000759 (2009)
  52. Terabayashi, T., Funato, Y., Fukuda, M., and Miki, H.
    A coated vesicle-associated kinase of 104 kDa (CVAK104) induces lysosomal degradation of frizzled 5 (Fzd5).
    J. Biol. Chem. 284, 26716-26724 (2009)
  53. Kise, Y., Morinaka, A., Teglund, S., and Miki, H.
    Sufu recruits GSK3beta for efficient processing of Gli3.
    Biochem. Biophys. Res. Commun. 387, 569-574 (2009)
  54. Terabayashi, T., Funato, Y., and Miki, H.
    Dishevelled-induced phosphorylation regulates membrane localization of Par1b.
    Biochem. Biophys. Res. Commun. 375, 660-665 (2008)
  55. Funato, Y., Michiue, T., Terabayashi, T., Yukita, A., Danno, H., Asashima, M., and Miki, H.
    Nucleoredoxin regulates the Wnt/planar cell polarity pathway in Xenopus.
    Genes. Cells. 13, 965-975 (2008)
  56. Terabayashi, T., Itoh, T. J., Yamaguchi, H., Yoshimura, Y., Funato, Y., Ohno, S., and Miki, H.
    Polarity-regulating kinase partitioning-defective 1/microtubule affinity-regulating kinase 2 negatively regulates development of dendrites on hippocampal neurons.
    J. Neurosci. 27,13098-13107 (2007)
  57. Funato, Y., and Miki, H.
    Nucleoredoxin, a novel thioredoxin family member involved in cell growth and differentiation.
    Antioxid. Redox Signal. 9, 1035-1057 (2007)
  58. Takenaka, K., Kise, Y., and Miki, H.
    GSK3beta positively regulates Hedgehog signaling through Sufu in mammalian cells.
    Biochem. Biophys. Res. Commun. 353, 501-508 (2007)
  59. Tsuchiya, D., Kitamura, Y., Takata, K., Taniguchi, T., Uemura, K., Miki, H., Takenawa, T., and Shimohama, S.
    Morphological Change by Overexpression of D385A Dominant Negative Presenilin 1 in Human Neuroblastoma SH-SY5Y Cells.
    J. Pharmacol. Sci. 102, 354-358 (2006)
  60. Kise, Y., Takenaka, K., Tezuka, T., Yamamoto, T., and Miki, H.
    Fused kinase is stabilized by Cdc37/Hsp90 and enhances Gli protein levels.
    Biochem. Biophys. Res. Commun. 351, 78-84 (2006)
  61. Tsuchiya, D., Kitamura, Y., Takata, K., Sugisaki, T., Taniguchi, T., Uemura, K., Miki, H., Takenawa, T., and Shimohama, S.
    Developmental expression of neural Wiskott-Aldrich syndrome protein (N-WASP) and WASP family verprolin-homologous protein
    (WAVE)-related proteins in postnatal rat cerebral cortex and hippocampus.
    Neurosci. Res. 56, 459-469 (2006)
  62. Funato, Y., Michiue, T., Asashima, M., and Miki, H.
    The thioredoxin-related redox-regulating protein nucleoredoxin inhibits Wnt-β-catenin signaling through Dishevelled.
    Nat. Cell Biol. 8, 501-508 (2006)
  63. Oda, A., Miki, H., Wada, I., Yamaguchi, H., Yamazaki, D., Suetsugu, S., Nakajima, M., Nakayama, A., Okawa, K., Miyazaki, H., Matsuno, K., Ochs, H. D., Machesky, L. M., Fujita, H., and Takenawa, T.
    WAVE/Scars in Platelets.
    Blood 105, 3141-3148 (2005)
  64. Bierne, H., Miki, H., Innocenti, M., Scita, G., Gertler, F. B., Takenawa, T., and Cossart, P.
    WASP-related proteins, Abi1 and Ena/VASP are required for Listeria invasion induced by the Met receptor.
    J. Cell Sci. 118, 1537-1547 (2005)
  65. Yamaguchi, H., Lorenz, M., Kempiak, S., Sarmiento, C., Coniglio, S., Symons, M., Segall, J., Eddy, R., Miki, H., Takenawa, T., and Condeelis, J.
    Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin.
    J. Cell Biol. 168, 441-452 (2005)
  66. Kawamura, K., Takano, K., Suetsugu, S., Kurisu, S., Yamazaki, D., Miki, H., Takenawa, T., and Endo, T.
    N-WASP and WAVE2 acting downstream of phosphatidylinositol 3-kinase are required for myogenic cell migration induced by hepatocyte growth factor
    J. Biol. Chem. 279, 54862-54871 (2004)
  67. Verma, S., Shewan, A. M., Scott, J. A., Helwani, F. M., Den Elzen, N. R., Miki, H., Takenawa, T., and Yap, A. S.
    Arp2/3 activity is necessary for efficient formation of E-cadherin adhesive contacts.
    J. Biol. Chem. 279, 34062-34070 (2004)
  68. Funato, Y., Terabayashi, T., Suenaga, N., Seiki, M., Takenawa, T., and Miki, H.
    IRSp53/Eps8 complex is important for positive regulation of Rac and cancer cell motility/invasiveness.
    Cancer Res. 64, 5237-5244 (2004)
  69. Takenaka, K., Nakagawa, H., Miyamoto, S., and Miki, H.
    The pre-mRNA splicing factor SF3a66 functions as a microtubule binding and bundling protein.
    Biochem. J. 382, 223-230 (2004)
  70. Miki, H., and Takenawa, T.
    Regulation of actin dynamics by WASP family proteins.
    J. Biochem. 134, 309-313 (2003)
  71. Yamazaki, D., Suetsugu, S., Miki, H., Kataoka, Y., Nishikawa, S., Fujiwara, T., Yoshida, N., and Takenawa, T.
    WAVE2 is required for directed cell migration and cardiovascular development.
    Nature 424, 452-456 (2003)
  72. Kitamura, Y., Tsuchiya, D., Takata, K., Shibagaki, K., Taniguchi, T., Smith, M. A., Perry, G., Miki, H., Takenawa, T., and Shimohama, S.
    Possible involvement of Wiskott-Aldrich syndrome protein family in aberrant neuronal sprouting in Alzheimer's disease.
    Neurosci. Lett. 346, 149-152 (2003)
  73. Kitamura, Y., Shibagaki, K., Takata, K., Tsuchiya, D., Taniguchi, T,. Gebicke-Haerter, P. J., Miki, H., Takenawa, T., and Shimohama, S
    Involvement of Wiskott-Aldrich syndrome protein family verprolin-homologous protein (WAVE) and Rac1 in the phagocytosis of amyloid-beta(1 - 42) in rat microglia.
    J. Pharmacol. Sci. 92, 115-123 (2003)
  74. Nakagawa, H., Miki, H., Nozumi, M., Takenawa, T., Miyamoto, S., Wehland, J., and Small, J. V.
    IRSp53 is colocalised with WAVE2 at the tips of protruding lamellipodia and filopodia independently of Mena.
    J.Cell Sci. 116, 2577-2583 (2003)
  75. Sawa, M., Suetsugu, S., Sugimoto, A., Miki, H., Yamamoto, M., and Takenawa, T.
    Essential role of the C. elegans Arp2/3 complex in cell migration during ventral enclosure.
    J. Cell Sci. 116, 1505-1518 (2003)
  76. Sun, P., Yamamoto, H., Suetsugu, S., Miki, H., Takenawa, T., and Endo, T.
    Small GTPase Rah/Rab34 is associated with membrane ruffles and macropinosomes and promotes macropinosome formation.
    J. Biol. Chem. 278, 4063-4071 (2003)
  77. Klein, C., Nguyen, D., Liu, C. H., Mizoguchi, A., Bhan, A. K., Miki, H., Takenawa, T., Rosen, F. S., Alt, F. W., Mulligan, R. C., and Snapper, S. B.
    Gene therapy for Wiskott Aldrich Syndrome: rescue of T-cell signaling and amelioration of colitis upon transplantation of retrovirally transduced hematopoietic stem cells in mice.
    Blood 101, 2159-2166 (2003)
  78. Nozumi, M., Nakagawa, H., Miki, H., Takenawa, T., and Miyamoto, S.
    Differential localization of WAVE isoforms in filopodia and lamellipodia of the neuronal growth cone.
    J. Cell Sci. 116, 239-246 (2003)
  79. Abe, T., Kato, M., Miki, H., Takenawa, T., and Endo, T.
    Small GTPase Tc10 and its homologue RhoT induce N-WASP-mediated long process formation and neurite outgrowth.
    J. Cell Sci. 116, 155-168 (2003)
  80. Suetsugu, S., Hattori, M., Miki, H., Tezuka, T., Yamamoto, T., Mikoshiba, K., and Takenawa, T.
    Sustained Activation of N-WASP through Phosphorylation Is Essential for Neurite Extension.
    Dev. Cell 3, 645-658 (2002).
  81. Yamaguchi, H., Miki, H., and Takenawa, T.
    Two verprolin homology domains increase the Arp2/3 complex-mediated actin polymerization activities of N-WASP and WAVE1 C-terminal regions.
    Biochem. Biophys. Res. Commun. 297, 214-219 (2002)
  82. Miki, H., and Takenawa, T.
    WAVE2 serves a functional partner of IRSp53 by regulating its interaction with Rac.
    Biochem. Biophys. Res. Commun. 293, 93-99 (2002)
  83. Yamaguchi, H., Miki, H., and Takenawa, T.
    Neural Wiskott-Aldrich syndrome protein is involved in hepatocyte growth factor-induced migration, invasion, and tubulogenesis of epithelial cells.
    Cancer Res. 62, 2503-2509 (2002)
  84. Suzuki, T., Mimuro, H., Suetsugu, S., Miki, H., Takenawa, T., and Sasakawa, C.
    Neural Wiskott-Aldrich syndrome protein (N-WASP) is the specific ligand for Shigella VirG among the WASP family and determines the host cell type allowing actin-based spreading.
    Cell. Microbiol. 4, 223-233 (2002)
  85. Suetsugu, S., Miki, H., and Takenawa, T.
    Spatial and temporal regulation of actin polymerization for cytoskeleton formation through Arp2/3 complex and WASP/WAVE proteins.
    Cell Motil. Cytoskeleton. 51, 113-122 (2002)
  86. Mizutani, K., Miki, H., He, H., Maruta, H., and Takenawa, T.
    Essential role of neural Wiskott-Aldrich syndrome protein in podosome formation and degradation of extracellular matrix in src-transformed fibroblasts.
    Cancer Res. 62, 669-674 (2002)
  87. Kato, M., Miki, H., Kurita, S., Endo, T., Nakagawa, H., Miyamoto, S., and Takenawa, T.
    WICH, a novel verprolin homology domain-containing protein that functions cooperatively with N-WASP in actin-microspike formation.
    Biochem. Biophys. Res. Commun. 291, 41-47 (2002)
  88. Vetterkind, S., Miki, H., Takenawa, T., Klawitz, I., Scheidtmann, K. H., and Preuss, U.
    The rat homologue of Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) associates with actin filaments, recruits N-WASP from the nucleus, and mediates mobilization of actin from stress fibers in favor of filopodia formation.
    J. Biol. Chem. 277, 87-95 (2002)
  89. Suetsugu, S., Miki, H., Yamaguchi, H., Obinata, T., and Takenawa, T.
    Enhancement of branching efficiency by the actin filament-binding activity of N-WASP/WAVE2.
    J. Cell Sci. 114, 4533-4542 (2001)
  90. Shcherbina, A., Miki, H., Kenney, D. M., Rosen, F. S., Takenawa, T., and Remold-O'Donnell, E.
    WASP and N-WASP in human platelets differ in sensitivity to protease calpain.
    Blood 98, 2988-2991 (2001)
  91. Suetsugu, S., Miki, H., and Takenawa, T
    Identification of another Actin-related protein (Arp) 2/3 complex binding site in Neural-Aldrich syndrome protein (N-WASP), that complements actin polymerization induced by the Arp2/3 complex activating (VCA) domain of N-WASP.
    J. Biol. Chem. 276, 33175-33180 (2001)
  92. Suetsugu, S., Miki, H., Yamaguchi, H., and Takenawa, T.
    Requirement of the basic region of N-WASP/WAVE2 for actin-based motility.
    Biochem. Biophys. Res. Commun. 282, 739-744 (2001)
  93. Takenawa, T., and Miki, H.
    WASP and WAVE family proteins: key molecules for rapid rearrangement of cortical actin filaments and cell movement.
    J. Cell Sci. 114, 1801-1809 (2001)
  94. Martinez-Quiles, N., Rohatgi, R., Anton, I. M., Medina, M., Saville, P., Miki, H., Yamaguchi, H., Takenawa, T., Hartwig, J. H., Geha, R. S., and Ramesh, N.
    WIP regulates N-WASP-mediated actin polymerization and filopodium formation.
    Nat. Cell Biol. 3, 484-491 (2001)
  95. Nakagawa, H., Miki, H., Ito, M., Ohashi, K., Takenawa, T., and Miyamoto, S.
    N-WASP, WAVE and Mena different roles in the organization of actin cytoskeleton in lamellipodia.
    J. Cell Sci. 114, 1555-1565 (2001)
  96. Fukuoka, M., Suetsugu, S., Miki, H., Fukami, K., Endo, T., and Takenawa, T.
    A Novel Neural Wiskott-Aldrich Syndrome Protein (N-WASP) Binding Protein, WISH, Induces Arp2/3 Complex Activation Independent of Cdc42.
    J. Cell Biol. 152, 471-482 (2001)
  97. Miki, H., Yamaguchi, H., Suetsugu, S., and Takenawa, T
    IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling.
    Nature 408, 732-735 (2000)
  98. Yamaguchi, H., Miki, H., Suetsugu, S., Ma, L., Kirschner, M. W., and Takenawa, T.
    Two tandem verprolin homology domains are necessary for a strong activation of Arp2/3 complex-induced actin polymerization and induction of microspike formation by N-WASP.
    Proc. Natl. Acad. Sci. USA 97, 12631-12636 (2000)
  99. Yang, C., Huang, M., DeBiasio, J., Pring, M., Joyce, M., Miki, H., Takenawa, T., and Zigmond, S. H.
    Profilin enhances Cdc42-induced nucleation of actin polymerization.
    J. Cell Biol. 150, 1001-1012 (2000)
  100. Mimuro, H., Suzuki, T., Suetsugu, S., Miki, H., Ttakenawa, T. and Sasakawa C.
    Profilin is required for sustaining efficient intra- and intercellular spreading of shigella flexneri.
    J. Biol. Chem. 275, 28893-28901 (2000)
  101. Suzuki, T., Mimuro, H., Miki, H., Takenawa, T., Sasaki, T., Nakanishi, H., Takai, Y., and Sasakawa, C.
    Rho Family GTPase Cdc42 Is Essential for the Actin-based Motility of Shigella in Mammalian Cells.
    J. Exp. Med. 191, 1905-1920 (2000)
  102. Sasaki, N., Miki, H., and Takenawa, T
    Arp2/3 Complex-Independent Actin Regulatory Function of WAVE.
    Biochem. Biophys. Res. Commun. 272, 386-390 (2000)
  103. Banzai, Y., Miki, H., Yamaguchi, H., and Takenawa, T.
    Essential role of neural Wiskott-Aldrich syndrome protein in neurite extension in PC12 cells and rat hippocampal primary culture cells.
    J. Biol. Chem. 275, 11987-11992 (2000)
  104. Miki, H., Fukuda, M., Nishida, E., and Takenawa, T.
    Phosphorylation of WAVE downstream of mitogen- activated protein kinase signaling.
    J. Biol. Chem. 274, 27605-27609 (1999)
  105. Kato, M., Miki, H., Imai, K., Nonoyama, S., Suzuki, T., Sasakawa, C., and Takenawa, T.
    Wiskott-Aldrich syndrome protein induces actin clustering without direct binding to Cdc42.
    J. Biol. Chem. 274, 27225-27230 (1999)
  106. Suetsugu, S., Miki, H., and Takenawa, T.
    Distinct roles of profilin in cell morphological changes: microspikes, membrane ruffles, stress fibers, and cytokinesis.
    FEBS Lett. 457, 470-474 (1999)
  107. Imai, K., Nonoyama, S., Miki, H., Morio, T., Fukami, K., Zhu, Q., Aruffo, A., Ochs, H. D., Yata, J., and Takenawa, T.
    The pleckstrin homology domain of the Wiskott-Aldrich syndrome protein is involved in the organization of actin cytoskeleton.
    Clin. Immunol. 92, 128-137 (1999)
  108. Suetsugu, S., Miki, H., and Takenawa, T
    Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex.
    Biochem. Biophys. Res. Commun. 260, 296-302 (1999)
  109. Rohatgi, R., Ma, L., Miki, H., Lopez, M., Kirchhausen, T., Takenawa, T., and Kirschner, M. W.
    The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly.
    Cell 97, 221-231 (1999)
  110. Miki, H., Suetsugu, S., and Takenawa, T.
    WAVE, a novel WASP-family protein involved in actin-reorganization induced by Rac.
    EMBO J. 17, 6932-6941 (1998)
  111. Suetsugu, S., Miki, H., and Takenawa, T.
    The essential role of profilin in the assembly of actin for microspike formation.
    EMBO J. 17, 6516-6526 (1998)
  112. Suzuki, T., Miki, H., Takenawa, T., and Sasakawa, C.
    Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri.
    EMBO J. 17, 2767-2776 (1998)
  113. Miki, H., and Takenawa, T.
    Direct binding of verprolin-homology domain of N-WASP to actin, which is essential for cytoskeletal reorganization.
    Biochem. Biophys. Res. Commun. 243, 73-78 (1998)
  114. Takenawa, T., Miki, H., and Matuoka, K.
    Signaling through Grb2/Ash-Control of the Ras pathway and the cytoskeleton.
    Curr. Top. Microbiol. Immunol. 228, 325-342 (1998)
  115. Miki, H., Sasaki, T., Takai, Y., and Takenawa, T.
    Induction of filopodium formation by a WASP-related actin-depolymerizing protein N-WASP.
    Nature 391, 93-96 (1998)
  116. Matuoka, K., Miki, H., Takahashi, K., and Takenawa, T.
    A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motifs.
    Biochem. Biophys. Res. Commun. 239, 488-492 (1997)
  117. Fukuoka, M., Miki, H., and Takenawa, T
    Identification of N-WASP homologs in human and rat brain.
    Gene 196, 43-48 (1997)
  118. Miki, H., Nonoyama, S., Zhu, Q., Aruffo, A., Ochs, H. D., Takenawa, T.
    A tyrosine kinase signalling regulates WASP function, which is essential for megakaryocyte differentiation.
    Cell Growth. Differ. 8, 195-202 (1997)
  119. Itoh, T., Miura, K.,Miki, H., and Takenawa, T.
    β-tubulin binds src homology 2 domains through a region different from the tyrosine-phosphorylated protein- recognizing site.
    J. Biol. Chem. 271, 27931-27935 (1996)
  120. Miki, H., Miura, K., and Takenawa, T.
    N-WASP, a novel actin-depolymerizing protein, regulates cortical cytoskeletal rearrangement in a PIP2-dependent manner downstream of tyrosine kinases.
    EMBO J. 15, 5326-5335 (1996)
  121. Takenawa, T., Miura, K., Miki, H., and Watanabe, K.
    Signal transductions of SH2/SH3: Ash/Grb-2 downstream signaling.
    Adv. in Pharmacol. 36, 139-153 (1996)
  122. Miura, K., Miki, H., Shimazaki, K., Kawai, N., and Takenawa, T.
    Interaction of ash/Grb-2 via its SH3 domains with neuron-specific p150 and p65.
    Biochem. J. 316, 639-645 (1996)
  123. Watanabe, K., Fukuchi, T., Hosoya, H., Shirasawa, T., Matuoka, K., Miki, H., and Takenawa, T.
    Splicing isoforms of rat Ash/Grb2.
    J. Biol. Chem. 270, 13733-13739 (1995)
  124. Nakanishi, H., Orita, S., Kaibuchi, K., Miura, K., Miki, H., Takenawa, T., and Takai, Y.
    Kinetic properties of Ash/Grb2-interacting GDP/GTP exchange protein.
    Biochem. Biophys. Res. Commun. 198, 1255-1261 (1994)
  125. Miki, H., Miura, K., Matuoka, K., Nakata, T., Hirokawa, N., Orita, S., Kaibuchi, K., Takai, Y., and Takenawa, T.
    Association of Ash/Grb-2 with dynamin through the src homology 3 domain.
    J. Biol. Chem. 269, 5489-5492 (1994)
PAGETOP