船戸 洋佑 Yosuke Funato

船戸 洋佑 / Yosuke Funato

准教授

船戸 洋佑
Yosuke Funato

学歴

2003　東京大学薬学部薬学科卒業
2008　同大学院修了、博士（薬学）

職歴

2005 - 2008　日本学術振興会特別研究員（DC1）
2008 - 2011　大阪大学蛋白質研究所・助教
2011 - 2022　大阪大学微生物病研究所・助教
2022 - 2023　大阪大学微生物病研究所・准教授
2023 - 現在　 京都大学大学院工学研究科・准教授
2022 - 現在　 JST創発的研究支援事業・創発研究者（兼任）

特記事項

2010　第62回日本細胞生物学会・若手優秀発表賞受賞
2010　大阪大学「飛翔30」若手プログラム・研究フェロー

研究業績

  1. #Ma, J., #Song, X., #Funato, Y., Teng, X., Huang, Y., Miki, H., Wang, W., and Hattori, M. (#: equal contribution)
    AI-driven mechanistic analysis of conformational dynamics in CNNM/CorC Mg2+ transporters.
    Structure in press
  2. #Funato, Y., Mimura, M., Nunomura, K., Lin, B., Fujii, S., Haruta, J., and #Miki, H. (#: co-correspondence)
    Development of a high-throughput screening system targeting the protein-protein interactions between PRL and CNNM.
    Sci. Rep. 14, 25432 (2024)
  3. Hashizume, O., Kawabe T., Funato, Y., and Miki, H.
    Intestinal Mg2+ accumulation induced by cnnm mutations decreases the body size by suppressing TORC2 signaling in Caenorhabditis elegans.
    Dev. Biol. 509, 59-69 (2024)
  4. #Funato, Y., Hashizume, O., and #Miki, H.(#: co-correspondence)
    Phosphatase-independent role of phosphatase of regenerating liver in cancer progression.
    Cancer Sci. 114, 25-33 (2023)
  5. Ryu, K., Yoshida, A., Funato, Y., Yamazaki, D., and Miki, H.
    PRL stimulates mitotic errors by suppressing kinetochore-localized activation of AMPK during mitosis.
    Cell Struct. Funct. 47, 75-87 (2022)
  6. Lohani, S., Funato, Y., Akieda, Y., Mizutani, K., Takai, Y., Ishitani, T., and Miki, H.
    A novel role for PRL in regulating epithelial cell density by inducing apoptosis at confluence.
    J. Cell Sci. 135, jcs258550 (2022)
  7. #Funato, Y. and #Miki, H.(#: co-correspondence)
    The emerging roles and therapeutic potential of cyclin M/CorC family of Mg2+ transporters.
    J. Pharmacol. Sci. 148, 14-18 (2022)
  8. Funato, Y., Yamazaki, D., Okuzaki, D., Yamamoto, N., and Miki, H.
    Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure.
    Nat. Commun. 12, 3683 (2021)
  9. Huang, Y., Mu, K., Teng, X., Zhao, Y., Funato, Y., Miki, H., Zhu, W., Xu, Z., and Hattori, M.
    Identification and mechanistic analysis of an inhibitor of the CorC Mg2+ transporter.
    iScience 24, 102370 (2021)
  10. Yamazaki, D., Hashizume, O., Taniguchi, S., Funato, Y., and Miki, H.
    Role of adenomatous polyposis coli in proliferation and differentiation of colon epithelial cells in organoid culture.
    Sci. Rep. 11, 3980 (2021)
  11. #Huang, Y., #Jin, F., #Funato, Y., Xu, Z., Zhu, W., Wang, J., Sun, M., Zhao, Y., Yu, Y., Miki, H., and Hattori, M. (#: equal contribution)
    Structural basis for the Mg2+ recognition and regulation of the CorC Mg2+ transporter.
    Science Advances 7, eabe6140 (2021)
  12. Wu, Y., Funato, Y., Meschi, E., Jovanoski, K., Miki, H., and Waddell, S.
    Magnesium efflux from Drosophila Kenyon Cells is critical for normal and diet-enhanced long-term memory.
    eLife 9, e61339 (2020)
  13. #Funato, Y., Yoshida, A., Hirata, Y., Hashizume, O., Yamazaki, D., and #Miki, H.(#: co-correspondence)
    The oncogenic PRL protein causes acid addiction of cells by stimulating lysosomal exocytosis.
    Dev. Cell 55, 387-397 (2020)
  14. #Kozlov, G., #Funato, Y., Chen, Y.S., Zhang, Z., Illes, K., Miki, H., and Gehring, K. (#: equal contribution)
    PRL3 pseudophosphatase activity is necessary and sufficient to promote metastatic growth.
    J. Biol. Chem. 295, 11682-11692 (2020)
  15. Hashizume, O., #Funato, Y., Yamazaki, D., and #Miki, H.(#: co-correspondence)
    Excessive Mg2+ Impairs Intestinal Homeostasis by Enhanced Production of Adenosine Triphosphate and Reactive Oxygen Species.
    Antioxid. Redox Signal. 33, 20-34 (2020)
  16. Dóka, É., Ida, T., Dagnell, M., Abiko, Y., Luong, N.C., Balog, N., Takata, T., Espinosa, B., Nishimura, A., Cheng, Q., Funato, Y., Miki, H., Fukuto, J.M., Prigge, J.R., Schmidt, E.E., Arnér, E.S.J., Kumagai, Y., Akaike, T., and Nagy, P.
    Control of protein function through oxidation and reduction of persulfidated states.
    Science Advances 6, eaax8358 (2020)
  17. Kojima, T., #Funato, Y., and #Miki, H.(#: co-correspondence)
    Phosphatase of regenerating liver sensitizes MET to functional activation by hepatocyte growth factor.
    Biochem. J. 476, 1419-1431 (2019)
  18. Yamazaki, D., Hasegawa, A., Funato, Y., Tran, H.N., Mori, M.X., Mori, Y., Sato, T., and Miki, H.
    Cnnm4 deficiency suppresses Ca2+ signaling and promotes cell proliferation in the colon epithelia.
    Oncogene 38, 3962-3969 (2019)
  19. Funato, Y. and Miki, H.
    Molecular function and biological importance of CNNM family Mg2+ transporters.
    J. Biochem. 165, 219-225 (2019)
  20. Chen, Y.S., Kozlov, G., Fakih, R., Funato, Y., Miki, H., and Gehring, K.
    The cyclic nucleotide-binding homology domain of the integral membrane protein CNNM mediates dimerization and is required for Mg2+ efflux activity.
    J. Biol. Chem. 293, 19998-20007 (2018)
  21. Yoshida, A., Funato, Y., and Miki, H.
    Phosphatase of regenerating liver maintains cellular magnesium homeostasis.
    Biochem. J. 475, 1129-1139 (2018)
  22. #Funato, Y., Furutani, K., Kurachi, Y., and #Miki, H.(#: co-correspondence)
    CNNM proteins are Na+/Mg2+ exchangers playing a central role in transepithelial Mg2+ (re)absorption.
    J. Physiol. 596, 743-746 (2018)
  23. Matsui, Y., Funato, Y., Imamura, H., Miki, H., Mizukami, S., and Kikuchi, K.
    Visualization of long-term Mg2+ dynamics in apoptotic cells using a novel targetable fluorescent probe.
    Chem. Sci. 8, 8255-8264 (2017)
  24. Funato, Y., Yamazaki, D., and Miki, H.
    Renal function of cyclin M2 Mg2+ transporter maintains blood pressure.
    J. Hypertens. 35, 585-592 (2017)
  25. #Gulerez, I., #Funato, Y., #Wu, H., Yang, M., Kozlov, G., Miki, H., and Gehring, K. (#: equal contribution)
    Phosphocysteine in the PRL-CNNM pathway mediates magnesium homeostasis.
    EMBO Rep. 17, 1890-1900 (2016)
  26. Ishii, T., #Funato, Y., Hashizume, O., Yamazaki, D., Hirata, Y., Nishiwaki, K., Kono, N., Arai, H., and #Miki, H. (#: co-correspondence)
    Mg2+ Extrusion from Intestinal Epithelia by CNNM Proteins Is Essential for Gonadogenesis via AMPK-TORC1 Signaling in Caenorhabditis elegans.
    PLoS Genet. 12, e1006276 (2016)
  27. Yamazaki, D., Funato, Y., Miyata, H., Ikawa, M., and Miki, H.
    Complementary role of CNNM2 in sperm motility and Ca(2+) influx during capacitation.
    Biochem. Biophys. Res. Commun. 474, 441-446 (2016)
  28. Yamazaki, D., Miyata, H., Funato, Y., Fujihara, Y., Ikawa, M., and Miki, H.
    Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction.
    J. Cell Sci. 129, 1940-1949 (2016)
  29. Hirata, Y., Funato, Y., and Miki, H.
    Basolateral sorting of the Mg(2+) transporter CNNM4 requires interaction with AP-1A and AP-1B.
    Biochem. Biophys. Res. Commun. 455, 184-189 (2014)
  30. Funato, Y., Yamazaki, D., Mizukami, S., Du, L., Kikuchi, K., and Miki, H.
    Membrane protein CNNM4-dependent Mg2+ efflux suppresses tumor progression.
    J. Clin. Invest. 124, 5398-5410 (2014)
  31. Yugi, K., Kubota, H., Toyoshima, Y., Noguchi, R., Kawata, K., Komori, Y., Uda, S., Kunida, K., Tomizawa, Y., Funato, Y., Miki, H., Matsumoto, M., Nakayama, K.I., Kashikura, K., Endo, K., Ikeda, K., Soga, T., and Kuroda, S.
    Reconstruction of insulin signal flow from phosphoproteome and metabolome data.
    Cell Rep. 8, 1171–1183 (2014)
  32. Hirata, Y., Funato, Y., Takano, Y., and Miki, H.
    Mg2+-dependent interactions of ATP with the cystathionine-β-synthase (CBS) domains of a magnesium transporter.
    J. Biol. Chem. 289, 14731-14739 (2014)
  33. Funato, Y. and Miki, H.
    Reversible oxidation of PRL family protein-tyrosine phosphatases.
    Methods 65, 184-189 (2014)
  34. #Yamazaki, D., #Funato, Y., Miura, J., Sato, S., Toyosawa, S., Furutani, K., Kurachi, Y., Omori, Y., Furukawa, T., Tsuda, T., Kuwabata, S., Mizukami, S., Kikuchi, K., and Miki, H. (#: equal contribution)
    Basolateral Mg2+ Extrusion via CNNM4 Mediates Transcellular Mg2+ Transport across Epithelia: A Mouse Model.
    PLoS Genet. 9, e1003983 (2013)
  35. Funato, Y., Hayashi, T., Irino, Y., Takenawa, T., and Miki, H.
    Nucleoredoxin regulates glucose metabolism via phosphofructokinase 1.
    Biochem. Biophys. Res. Commun. 440, 737-742 (2013)
  36. Ishii, T., Funato, Y., and Miki, H.
    Thioredoxin-related protein 32 (TRP32) specifically reduces oxidized phosphatase of regenerating liver (PRL).
    J. Biol. Chem. 288, 7263–7270 (2013)
  37. Miki, H., and Funato, Y.
    Regulation of intracellular signalling through cysteine oxidation by reactive oxygen species.
    J. Biochem. 151, 255–261 (2012)
  38. Morinaka, A., Funato, Y., Uesugi, K., and Miki, H.
    Oligomeric peroxiredoxin-I is an essential intermediate for p53 to activate MST1 kinase and apoptosis.
    Oncogene 30, 4208-4218 (2011)
  39. Morinaka, A., Yamada, M., Itofusa, R., Funato, Y., Yoshimura, Y., Nakamura, F., Yoshimura, T., Kaibuchi, K., Goshima, Y., Hoshino, M., Kamiguchi, H., and Miki, H.
    Thioredoxin mediates oxidation-dependent phosphorylation of CRMP2 and growth cone collapse.
    Science Signal. 4, ra26 (2011)
  40. Müller, L., Funato, Y., Miki, H., and Zimmermann, R.
    An interaction between human Sec63 and nucleoredoxin may provide the missing link between the SEC63 gene and polycystic liver disease.
    FEBS Lett. 585, 596-600 (2011)
  41. Funato, Y., Terabayashi, T., Sakamoto, R., Okuzaki, D., Ichise, H., Nojima, H., Yoshida, N., and Miki, H.
    Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive dishevelled protein.
    Curr. Biol. 20, 1945-1952 (2010)
  42. #Hayashi, T., #Funato, Y., Tearabayashi, T., Morinaka, A., Sakamoto, R., Ichise, H., Fukuda, H., Yoshida, N., and Miki, H. (#: equal contribution)
    Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of Flightless-I toMyeloid differentiation primary response gene (88).
    J. Biol. Chem. 285, 18586-18593 (2010)
  43. #Funato, Y., and #Miki, H. (#: co-correspondence)
    Redox regulation of Wnt signalling via nucleoredoxin.
    Free Radic. Res. 44, 379-388 (2010)
  44. Boles, M. K., Wilkinson, B. M., Wilming, L., Liu, B., Probst, F. J., Harrow, J., Grafham, D., Hentges, K. E., Woodward, L. P., Maxwell, A., Mitchell, K., Risley, M. D., Johnson, R., Hirschi, K., Lupski, J. R., Funato, Y., Miki, H., Marcin-Garcia, P., Mattews, L., Coffey, A. J., Parker, A., Hubbard, T. J., Rogers, J., Bradley, A., Adams, D. J., and Justice, M. J.
    Discovery of candidate disease genes in ENU-induced mouse mutants by large-scale sequencing identifies a splice-site mutation in nucleoredoxin.
    PLoS Genet. 5, e1000759 (2009)
  45. Terabayashi, T., Funato, Y., Fukuda, M., and Miki, H.
    A coated vesicle-associated kinase of 104 kDa (CVAK104) induces lysosomal degradation of frizzled 5 (Fzd5).
    J. Biol. Chem. 284, 26716-26724 (2009)
  46. Terabayashi, T., Funato, Y., and Miki, H.
    Dishevelled-induced phosphorylation regulates membrane localization of Par1b.
    Biochem. Biophys. Res. Commun. 375, 660-665 (2008)
  47. Funato, Y., Michiue, T., Terabayashi, T., Yukita, A., Danno, H., Asashima, M., andMiki, H.
    Nucleoredoxin regulates the Wnt/planar cell polarity pathway in Xenopus.
    Genes. Cells. 13, 965-975 (2008)
  48. Terabayashi, T., Itoh, T. J., Yamaguchi, H., Yoshimura, Y., Funato, Y., Ohno, S., and Miki, H.
    Polarity-regulating kinase partitioning-defective 1/microtubule affinity-regulating kinase 2 negatively regulates development of dendrites on hippocampal neurons.
    J. Neurosci. 27,13098-13107 (2007)
  49. Funato, Y., and Miki, H.
    Nucleoredoxin, a novel thioredoxin family member involved in cell growth and differentiation.
    Antioxid. Redox Signal. 9, 1035-1057 (2007)
  50. Funato, Y., Michiue, T., Asashima, M., and Miki, H.
    The thioredoxin-related redox-regulating protein nucleoredoxin inhibits Wnt-β-catenin signaling through Dishevelled.
    Nat. Cell Biol. 8, 501-508 (2006)
  51. Funato, Y., Terabayashi, T., Suenaga, N., Seiki, M., Takenawa, T., and Miki, H.
    IRSp53/Eps8 complex is important for positive regulation of Rac and cancer cell motility/invasiveness.
    Cancer Res. 64, 5237-5244 (2004)
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